pET28a(+)-Gx-SB
(Plasmid
#220125)
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PurposeExpresses the 1.3-kDa Small BiT (SB) fragment of split NanoLuc, genetically fused via a semi-rigid peptide linker to a protein G adapter domain (Gx).
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220125 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a(+)
- Backbone size w/o insert (bp) 5242
- Total vector size (bp) 5749
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGx-SB
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Insert Size (bp)507
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a(+)-Gx-SB was a gift from Maarten Merkx (Addgene plasmid # 220125 ; http://n2t.net/addgene:220125 ; RRID:Addgene_220125) -
For your References section:
A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays. Ni Y, Rosier BJHM, van Aalen EA, Hanckmann ETL, Biewenga L, Pistikou AM, Timmermans B, Vu C, Roos S, Arts R, Li W, de Greef TFA, van Borren MMGJ, van Kuppeveld FJM, Bosch BJ, Merkx M. Nat Commun. 2021 Jul 28;12(1):4586. doi: 10.1038/s41467-021-24874-3. 10.1038/s41467-021-24874-3 PubMed 34321486
Map uploaded by the depositor.
