LLhPtxS-pHG165a
(Plasmid
#220271)
-
PurposeConstitutive expression of a chimeric LacI:PtxS transcription repressor
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 220271 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepHG165a
-
Backbone manufacturerJonathan Kuhn (Stewart et al. 1986 PMID: 3012611)
-
Modifications to backboneThe residual LacI C-terminal codons at a second locus in the original pHG165 vector were removed to facilitate mutagenesis.
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameLLhPtxS
-
Alt nameLacI:PtxS transcription repressor
-
SpeciesSynthetic
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
LLhPtxS-pHG165a was a gift from Liskin Swint-Kruse (Addgene plasmid # 220271 ; http://n2t.net/addgene:220271 ; RRID:Addgene_220271)