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Addgene

pET-15b-aδNb367
(Plasmid #220320)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220320 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5576
  • Total vector size (bp) 6050
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    aδNb367
  • Alt name
    anti-IgD nanobody 367
  • Species
    Synthetic
  • Insert Size (bp)
    474
  • Promoter T7
  • Tags / Fusion Proteins
    • TEV protease cleavage site (C terminal on insert)
    • His6 (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-15b-aδNb367 was a gift from James McDonnell (Addgene plasmid # 220320 ; http://n2t.net/addgene:220320 ; RRID:Addgene_220320)

  • For your References section:

    Anti-IgD nanobodies as novel tools for studying human IgD biology. Vester SK, Beavil RL, Alexandrovich A, Gould HJ, Beavil AJ, Sutton BJ, McDonnell JM. Sci Rep. 2025 Jul 8;15(1):24455. doi: 10.1038/s41598-025-09118-4. 10.1038/s41598-025-09118-4 PubMed 40628825
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