pFSW-BsmBI-shRNA-hSyn-GFP
(Plasmid
#220436)
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Purpose(Empty Backbone) Lentiviral shRNA cloning backbone with GFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220436 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFSW
- Backbone size (bp) 8000
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Vector typeMammalian Expression, Lentiviral, RNAi
- Promoter U6
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer LKO.1 5' (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFSW-BsmBI-shRNA-hSyn-GFP was a gift from Paul Blainey (Addgene plasmid # 220436 ; http://n2t.net/addgene:220436 ; RRID:Addgene_220436) -
For your References section:
High-content image-based pooled screens reveal regulators of synaptogenesis. Le A, Biederer T, Blainey PC. Cell Rep. 2025 Jun 24;44(7):115889. doi: 10.1016/j.celrep.2025.115889. 10.1016/j.celrep.2025.115889 PubMed 40560725