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Addgene

PhoCoil
(Plasmid #220444)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220444 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET21
  • Backbone size w/o insert (bp) 5368
  • Total vector size (bp) 7069
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PhoCoil
  • Species
    Synthetic
  • Insert Size (bp)
    1701
  • Promoter T7
  • Tag / Fusion Protein
    • 6x His (N and C term)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PhoCoil was a gift from Cole DeForest (Addgene plasmid # 220444 ; http://n2t.net/addgene:220444 ; RRID:Addgene_220444)

  • For your References section:

    PhoCoil: A photodegradable and injectable single-component recombinant protein hydrogel for minimally invasive delivery and degradation. Gregorio NE, Zhang F, Suita Y, Ang LS, Prado O, Fasuyi A, Olson JM, Stevens KR, DeForest CA. Sci Adv. 2025 Aug 29;11(35):eadx3472. doi: 10.1126/sciadv.adx3472. Epub 2025 Aug 27. 10.1126/sciadv.adx3472 PubMed 40864698
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