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pAAV-Puro_siKD2.0
(Plasmid #220536)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220536 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAAV-Puro_sikD
  • Vector type
    Bacterial Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pAAV-Puro_siKD2.0 is an AAVS1-targeting vector designed for the iPS2-seq platform to express a single-copy, tetracycline-inducible shRNA in human pluripotent stem cells. Custom shRNAs are cloned into the vector together with a unique barcode (BC) and a random unique clonal identifier (UCI), enabling perturbation assignment and clonal tracking in single-cell RNA-seq. The plasmid includes a puromycin gene-trap cassette for positive selection after AAVS1 integration and is co-delivered with a filler plasmid (pAAV-Neo_CAG, Plasmid #220537) for efficient biallelic targeting.

Please visit https://dx.doi.org/10.17504/protocols.io.e6nvw4q29lmk/v1 for a detailed protocol for use.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Puro_siKD2.0 was a gift from Alessandro Bertero (Addgene plasmid # 220536 ; http://n2t.net/addgene:220536 ; RRID:Addgene_220536)

  • For your References section:

    Single Cell Transcriptional Perturbome in Pluripotent Stem Cell Models. Balmas E, Ratto ML, Snijders KE, Becca S, Liaci C, Ricca I, Merlo GR, Calogero RA, Alessandrì L, Mendjan S, Bertero A. Mol Syst Biol (2025). https://doi.org/10.1038/s44320-025-00172-8 10.1038/s44320-025-00172-8
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