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pAAV-Neo_CAG
(Plasmid #220537)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220537 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAAV-Neo_CAG
  • Vector type
    Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pAAV-Neo_CAG is an empty AAVS1-targeting backbone used as a filler plasmid during genome editing. It carries a CAG-driven neomycin resistance cassette and is co-delivered with pAAV-Puro_siKD2.0 (Plasmid #220536) during nucleofection to enrich for biallelic AAVS1 integration. This plasmid does not express any shRNA or functional payload and serves only as a selection-compatible filler vector.

Please visit https://dx.doi.org/10.17504/protocols.io.e6nvw4q29lmk/v1 for a detailed protocol for use.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Neo_CAG was a gift from Alessandro Bertero (Addgene plasmid # 220537 ; http://n2t.net/addgene:220537 ; RRID:Addgene_220537)
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