|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22060||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5100
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt namethymidine kinase
Insert Size (bp)1400
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (destroyed during cloning)
- 3′ cloning site SalI (destroyed during cloning)
- 5′ sequencing primer T7
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
Note: the TK sequence is not listed in the full plasmid sequence, although it was detected in Addgene's sequencing results.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS-MCS1M(1)Kana-mCMV-TK-pA was a gift from Maria Castro (Addgene plasmid # 22060 ; http://n2t.net/addgene:22060 ; RRID:Addgene_22060)
For your References section:High-capacity adenovirus vector-mediated anti-glioma gene therapy in the presence of systemic antiadenovirus immunity. King GD, Muhammad AK, Xiong W, Kroeger KM, Puntel M, Larocque D, Palmer D, Ng P, Lowenstein PR, Castro MG. J Virol. 2008 May . 82(9):4680-4. 10.1128/JVI.00232-08 PubMed 18287240