AiP2141 - pAAV-AiE2333m-minBG-iCre(R297T)-BGHpA
(Plasmid #220731)

• Purpose: AiE2333m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells
• Depositing Labs: The Allen Institute for Brain Science, Bosiljka Tasic
• Publication: Cortical Paper (TIGRE 3.0) (unpublished)

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Backbone

• Vector backbone: pAAV2
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: iCre(R297T)
• Species: Synthetic
• Promoter: minBG

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • AiP2141.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

5' Cloning Site: MluI (not destroyed), 3' Cloning Site: SgrAI (not destroyed).