AiP2141 - pAAV-AiE2333m-minBG-iCre(R297T)-BGHpA
(Plasmid
#220731)
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PurposeAiE2333m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220731 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV2
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiCre(R297T)
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SpeciesSynthetic
- Promoter minBG
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' Cloning Site: MluI (not destroyed), 3' Cloning Site: SgrAI (not destroyed).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AiP2141 - pAAV-AiE2333m-minBG-iCre(R297T)-BGHpA was a gift from The Allen Institute for Brain Science & Bosiljka Tasic (Addgene plasmid # 220731)