0A-1169
(Plasmid
#220935)
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PurposeA QUAS line that expresses VSR proteins in Ae. aegypti when crossed with a driver line, Opie-2 dsRed.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220935 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneOA-1144A
- Backbone size w/o insert (bp) 8066
- Total vector size (bp) 9401
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCricket paralysis virus (CrPV) 1A protein, Nodamura virus (NoV) B2 protein, and flock house virus (FHV) B2
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Insert Size (bp)1176
- Promoter hsp70
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 and T7 term, 1136H.S1~S4, 1146E.S1~ S6 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
0A-1169 was a gift from Omar Akbari (Addgene plasmid # 220935 ; http://n2t.net/addgene:220935 ; RRID:Addgene_220935)
Map uploaded by the depositor.
