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pdCas12f1Cas_ABE-LVA
(Plasmid #220995)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220995 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    p15a
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Acidibacillus sulfuroxidans Cas12f1-ABE
  • Species
    Acidibacillus sulfuroxidans, Escherichia coli
  • Mutation
    D225A
  • Tag / Fusion Protein
    • LVA (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pdCas12f1Cas_ABE-LVA was a gift from John van der Oost (Addgene plasmid # 220995 ; http://n2t.net/addgene:220995 ; RRID:Addgene_220995)

  • For your References section:

    Base editing both DNA strands in distinct editing windows with small CRISPR-associated effector Cas12f1. Swartjes T, Bouzetos E, Adiego-Perez B, Pool VD, Staals RHJ, van der Oost J, Wu WY. iScience. 2025 Nov 12;28(12):114033. doi: 10.1016/j.isci.2025.114033. eCollection 2025 Dec 19. 10.1016/j.isci.2025.114033 PubMed 41399500
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