pTarget-entry
(Plasmid
#220996)
-
PurposeProkaryotic expression of mRFP and GFP. Target sequences are inserted in place of the RFP gene through AatII/KpnI restriction ligation
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220996 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepSC101
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert namemRFP, GFP
-
SpeciesDiscosoma species, Aequorea victoria
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTarget-entry was a gift from John van der Oost (Addgene plasmid # 220996 ; http://n2t.net/addgene:220996 ; RRID:Addgene_220996) -
For your References section:
Base editing both DNA strands in distinct editing windows with small CRISPR-associated effector Cas12f1. Swartjes T, Bouzetos E, Adiego-Perez B, Pool VD, Staals RHJ, van der Oost J, Wu WY. iScience. 2025 Nov 12;28(12):114033. doi: 10.1016/j.isci.2025.114033. eCollection 2025 Dec 19. 10.1016/j.isci.2025.114033 PubMed 41399500