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Addgene

MSCV-KRAS-G12D-IRES-mCherry
(Plasmid #221022)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221022 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    MSCV-IRES-mCherry
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    KRAS G12D
  • Species
    H. sapiens (human)
  • Entrez Gene
    KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.09.26.557083 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-KRAS-G12D-IRES-mCherry was a gift from Mohamed Fareh (Addgene plasmid # 221022 ; http://n2t.net/addgene:221022 ; RRID:Addgene_221022)

  • For your References section:

    Principles of CRISPR-Cas13 mismatch intolerance enable selective silencing of point-mutated oncogenic RNA with single-base precision. Shembrey C, Yang R, Casan J, Hu W, Chen H, Singh GJ, Sadras T, Prasad K, Shortt J, Johnstone RW, Trapani JA, Ekert PG, Fareh M. Sci Adv. 2024 Dec 20;10(51):eadl0731. doi: 10.1126/sciadv.adl0731. Epub 2024 Dec 18. 10.1126/sciadv.adl0731 PubMed 39693429
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