Tg(Sox17::EMTB-3xGFP)
(Plasmid #221190)

• Purpose: To specifically label microtubules (MTs) in Kupffer's Vesicle (KV)
• Depositing Lab: Heidi Hehnly
• Depositing Organization: Syracuse University
  Located in United States of America
• Publication: Wu et al Development. 2025 May 15;152(10):dev204687. doi: 10.1242/dev.204687. Epub 2025 May 19.

Backbone

• Vector backbone: Custom Tol2 backbone (pUC57 with modified Tol2 R & L arms)
• Backbone manufacturer: Nemametrix, Inc (dba InVivo Biosystems)
• Backbone size w/o insert (bp): 3599
• Total vector size (bp): 12265
• Modifications to backbone: None
• Vector type: Zebrafish

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Ensconsin Microtubule-Binding Protein
• Alt name: EMTB
• Species: H. sapiens (human)
• Insert Size (bp): 2256
• Mutation: Microtubule binding-domain of ensconsin (A.A. 18-283)
• GenBank ID: LT734165.1
• Entrez Gene: MAP7 (a.k.a. E-MAP-115, EMAP115)
• Promoter: Sox17
• Tag / Fusion Protein:
  • 3 EGFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13R
• 3′ sequencing primer: M13F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2024.05.12.593765 for bioRxiv preprint.

DNA (Catalog # 221190-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 221190-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.