FHpHG_mTent5c
(Plasmid
#221440)
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PurposeTransfection into the cells for the reporter or qPCR assay.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221440 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDH_CMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTent5c
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SpeciesM. musculus (mouse)
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Entrez GeneFam46c (a.k.a. DN-384L23.3, 4930431B09Rik, AI449797)
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Tag
/ Fusion Protein
- FLAG-2xHA-(HRV 3C site)-6xHis-EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsiWI (unknown if destroyed)
- 3′ cloning site SacII (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FHpHG_mTent5c was a gift from Marcos Morgan (Addgene plasmid # 221440 ; http://n2t.net/addgene:221440 ; RRID:Addgene_221440) -
For your References section:
TENT5C functions as a corepressor in the ligand-bound glucocorticoid receptor and estrogen receptor alpha complexes. Li Y, Perera L, He RS, Baptissart M, Petrovich RM, Morgan M. FEBS J. 2025 May 27. doi: 10.1111/febs.70137. 10.1111/febs.70137 PubMed 40421654