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Addgene

pKANAC3K
(Plasmid #221545)

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Item Catalog # Description Quantity Price (USD)
Plasmid 221545 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pKANAC3K
  • Backbone size (bp) 9529
  • Vector type
    Activation tagging in plant
  • Promoter Cauliflower mosaic virus 35S
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Selection with ampicillin may result in many satellite colony formations, but selection with 100 mg/L carbenicillin may suppress satellite colony formation.
  • Copy number
    High Copy

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pVS1 StaA, pVS1 RepA, NeoR/KanR, and CaMV poly(A) signal were derived from pCAMBIA (CAMBIA org., Black Mountain, ACT, Australia). NOS promoter, AmpR, Ori, and 4x35S enhancer were derived from pPCVICEn4HPT (Peter Meyer. The University of Leeds, Leeds, UK). LB and RB T-DNA repeats were derived from pGKB5 (David Bouchez. I. N. R. A. Laboratoire de Biologie Cellulaire FRANCE).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKANAC3K was a gift from Keiichi Shimizu (Addgene plasmid # 221545 ; http://n2t.net/addgene:221545 ; RRID:Addgene_221545)
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