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pBS-HYGRO-P2A-mAID-mCLOVER-LRR1
(Plasmid #221546)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221546 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBluescript KS II+
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 6000
  • Modifications to backbone
    Homology arm was inserted as detailed
  • Vector type
    Unspecified
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LRR1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3000
  • Entrez Gene
    LRR1 (a.k.a. 4-1BBLRR, LRR-1, PPIL5)
  • Promoter N/A
  • Tag / Fusion Protein
    • HYGRO-P2A-mAID-mCLOVER (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SACI (unknown if destroyed)
  • 3′ cloning site KNPI (unknown if destroyed)
  • 5′ sequencing primer TCAACgagctcAGTTCTAAGTGCCTTTCCCTGTAAC
  • 3′ sequencing primer ACTCAggtaccCAGTTATTTTACTGTTCCCCAACTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-HYGRO-P2A-mAID-mCLOVER-LRR1 was a gift from Aga Gambus (Addgene plasmid # 221546 ; http://n2t.net/addgene:221546 ; RRID:Addgene_221546)

  • For your References section:

    Characterizing replisome disassembly in human cells. Jones RM, Ruiz JH, Scaramuzza S, Nath S, Liu C, Henklewska M, Natsume T, Bristow RG, Romero F, Kanemaki MT, Gambus A. iScience. 2024 Jun 12;27(7):110260. doi: 10.1016/j.isci.2024.110260. eCollection 2024 Jul 19. 10.1016/j.isci.2024.110260 PubMed 39055910
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