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Addgene

POC1428
(Plasmid #221575)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221575 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pICH47732
  • Backbone manufacturer
    Self-made
  • Backbone size w/o insert (bp) 4325
  • Total vector size (bp) 5175
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site
  • Insert Size (bp)
    850

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The DH10B strain should be used for expression of this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    POC1428 was a gift from Christopher A. O'Callaghan (Addgene plasmid # 221575 ; http://n2t.net/addgene:221575 ; RRID:Addgene_221575)
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