DN-28z-CAR-miSFIT-9-T
(Plasmid
#221627)
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PurposeTuning expression of DN-28z CAR with a microRNA response element in the 3'-UTR
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 221627 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepPD-1-miSFIT-4x
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Backbone manufacturerTudor Fulga, Addgene #124678
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Vector typeMammalian Expression, Affinity Reagent/ Antibody
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDN-28z chimeric antigen receptor (CAR)
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DN-28z-CAR-miSFIT-9-T was a gift from Tatjana Sauka-Spengler (Addgene plasmid # 221627 ; http://n2t.net/addgene:221627 ; RRID:Addgene_221627)