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mCherry-Rubicon
(Plasmid #221657)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221657 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pmCherryC1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7594
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RUBCN
  • Alt name
    Rubicon
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2919
  • GenBank ID
    9711
  • Entrez Gene
    RUBCN (a.k.a. KIAA0226, RUBICON, SCAR15)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrG1 (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer 5'-CACCTACAAGGCCAAGAAGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-Rubicon was a gift from Erika Holzbaur (Addgene plasmid # 221657 ; http://n2t.net/addgene:221657 ; RRID:Addgene_221657)

  • For your References section:

    Mitochondrial damage triggers the concerted degradation of negative regulators of neuronal autophagy. Basak B, Holzbaur ELF. Nat Commun. 2025 Aug 9;16(1):7367. doi: 10.1038/s41467-025-62379-5. 10.1038/s41467-025-62379-5 PubMed 40783388
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