pEGFP-C1_N-NLS
(Plasmid #221867)

• Purpose: (Empty Backbone) Empty backbone for expressing GFP-fusion proteins in mammalian cells with a nuclear localization signal (NLS).
• Depositing Lab: Roger Woodgate
• Publication: Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag098. doi: 10.1093/nar/gkag098.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size (bp): 4761
• Modifications to backbone: A nuclear localization signal (NLS) has been fused to the N-terminus of EGFP
• Vector type: Mammalian Expression
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Resource Information

• Supplemental Documents:
  • pEGFP-C1_N-NLS.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a variant of pEGFP-C1 (Clontech) that we modified by addition of an SV40 nuclear localization signal upstream of the EGFP coding sequence.

Please visit https://doi.org/10.1101/2024.10.12.618026 for bioRxiv preprint.