pHR-IDR3-mCh-Cry2WT-NLS
(Plasmid
#221919)
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PurposeExpression of CBP IDR3 fragment fused to optogenetic protein mCh-Cry2WT
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 221919 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepHr-mCh-Cry2WT
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Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 11303
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCBP IDR3 (aa 441-587)
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Alt nameCREB binding protein
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SpeciesH. sapiens (human)
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Insert Size (bp)441
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Mutationinsertion of the IDR3 region (aa 441 - 587); insertion of SV40 NLS
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Entrez GeneCREBBP (a.k.a. CBP, KAT3A, MKHK1, RSTS, RSTS1)
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR-IDR3-mCh-Cry2WT-NLS was a gift from Daniel Bose (Addgene plasmid # 221919 ; http://n2t.net/addgene:221919 ; RRID:Addgene_221919) -
For your References section:
CBP-IDRs regulate acetylation and gene expression. Gelder KL, Carruthers NA, Gilbert G, Harrison LJ, Evans BV, Evans TI, Ball SS, Dunning M, Craggs TD, Twelvetrees AE, Bose DA. Cell Rep. 2026 Mar 12;45(3):117109. doi: 10.1016/j.celrep.2026.117109. 10.1016/j.celrep.2026.117109 PubMed 41824454