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Addgene

pFastBac-M13-8xHis-ZZ-LTLT-HOOK3
(Plasmid #222287)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222287 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastbacM13
  • Total vector size (bp) 7463
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOOK3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2157
  • Entrez Gene
    HOOK3 (a.k.a. HK3)
  • Tags / Fusion Proteins
    • 8 His (N terminal on insert)
    • ZZ tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer ZZ tag fw
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.10.26.564242 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac-M13-8xHis-ZZ-LTLT-HOOK3 was a gift from Anne Straube (Addgene plasmid # 222287 ; http://n2t.net/addgene:222287 ; RRID:Addgene_222287)

  • For your References section:

    KIF1C activates and extends dynein movement through the FHF cargo adapter. Abid Ali F, Zwetsloot AJ, Stone CE, Morgan TE, Wademan RF, Carter AP, Straube A. Nat Struct Mol Biol. 2025 Apr;32(4):756-766. doi: 10.1038/s41594-024-01418-z. Epub 2025 Jan 2. 10.1038/s41594-024-01418-z PubMed 39747486
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