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pET22b-GST-6P2-LTLT-KIF1C(642–922)-6His
(Plasmid #222289)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222289 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET22b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KIF1C
  • Alt name
    SAX2
  • Alt name
    KIAA0706
  • Alt name
    LTXS1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    840
  • Mutation
    stalk region only amino acids 642 to 922
  • Entrez Gene
    KIF1C (a.k.a. LTXS1, SATX2, SAX2, SPAX2, SPG58)
  • Promoter T7
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • 6His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer GST forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.10.26.564242 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET22b-GST-6P2-LTLT-KIF1C(642–922)-6His was a gift from Anne Straube (Addgene plasmid # 222289 ; http://n2t.net/addgene:222289 ; RRID:Addgene_222289)

  • For your References section:

    KIF1C activates and extends dynein movement through the FHF cargo adapter. Abid Ali F, Zwetsloot AJ, Stone CE, Morgan TE, Wademan RF, Carter AP, Straube A. Nat Struct Mol Biol. 2025 Apr;32(4):756-766. doi: 10.1038/s41594-024-01418-z. Epub 2025 Jan 2. 10.1038/s41594-024-01418-z PubMed 39747486
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