GSE22 T329A in pENTR1A (w187-1)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22253||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2277
Vector typeEntry vector
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth instructionsTOP10F', 370C.
Copy numberHigh Copy
Gene/Insert nameGenetic suppressor element #22, T329A
Alt nameGSE22 T329A
SpeciesR. norvegicus (rat)
Insert Size (bp)245
MutationThreonine 329 is mutated to Alanine (p53 nomencature).
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (not destroyed)
- 3′ cloning site Xho I (destroyed during cloning)
- 5′ sequencing primer ENTRforw
- 3′ sequencing primer ENTRrev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byGSE22 was obtained from Dr.Christian Beausejour
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
GSE22 originally published in Ossovskaya et al. PNAS 93: 10309-10314. T329A mutation was shown to not affect tetramerization of p53 (Mateu et al. Nat Struct Biol. 1999 Feb;6(2):191-8).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GSE22 T329A in pENTR1A (w187-1) was a gift from Eric Campeau (Addgene plasmid # 22253 ; http://n2t.net/addgene:22253 ; RRID:Addgene_22253)