SV40 Large T K1 mutant in pENTR1A (w414-1)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22270||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2279
Vector typeEntry vector
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth instructionsTOP10F', 37oC
Copy numberHigh Copy
Gene/Insert nameSV40 Large T antigen TK1 mutant (E107K)
Insert Size (bp)2126
MutationE107K (K1 mutation)
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (destroyed during cloning)
- 3′ cloning site Xho I (destroyed during cloning)
- 5′ sequencing primer ENTRforw
- 3′ sequencing primer ENTRrev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byLarge T K1 was obtained from Dr. Francis Rodier.
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Large T antigen K1 mutant can no longer bind and inactivate the Rb, p130 and p107 proteins (DeCaprio et al. 1988, Stubdal et al. 1997). It also fails to activate Akt (Yu and Alwine 2008).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SV40 Large T K1 mutant in pENTR1A (w414-1) was a gift from Eric Campeau (Addgene plasmid # 22270 ; http://n2t.net/addgene:22270 ; RRID:Addgene_22270)