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pPB.DEST-Illusia(Y783F)
(Plasmid #222596)

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Item Catalog # Description Quantity Price (USD)
Plasmid 222596 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pPB.DEST
  • Total vector size (bp) 8104
  • Vector type
    Mammalian Expression ; Transposon-based stable expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Illusia(Y783F)
  • Species
    Synthetic
  • Insert Size (bp)
    2274
  • Mutation
    Mutation in the NPxY(783) site equivolent to Y783F in full-length integrin beta1 (ITGB1)
  • Promoter CAG

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB.DEST-Illusia(Y783F) was a gift from Johanna Ivaska (Addgene plasmid # 222596 ; http://n2t.net/addgene:222596 ; RRID:Addgene_222596)

  • For your References section:

    Dynamic regulation of integrin beta1 phosphorylation supports invasion of breast cancer cells. Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska J. Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4. 10.1038/s41556-025-01663-4 PubMed 40419795
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