pBOBI-hPLD4-flag
(Plasmid
#222641)
-
Purposelentiviral vector for full length human PLD4 expression (with flag tag)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 222641 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepBOBI
- Backbone size w/o insert (bp) 9000
- Total vector size (bp) 10500
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePLD4
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1500
-
GenBank ID
-
Entrez GenePLD4 (a.k.a. C14orf175)
- Promoter CMV
-
Tag
/ Fusion Protein
- flag (C terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBOBI-hPLD4-flag was a gift from David Nemazee (Addgene plasmid # 222641 ; http://n2t.net/addgene:222641 ; RRID:Addgene_222641)
