PAF24
(Plasmid
#223172)
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PurposeExpresses VC83_00191-yeGFP under control of CTR1 promoter and terminator
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223172 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS313
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCopper Transporter
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Alt nameCTR
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Alt nameVC83_00191
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SpeciesPseudogymnoascus destructans
- Promoter saccharomyces cerevisiae CTR1
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Tags
/ Fusion Proteins
- StreptagII (C terminal on insert)
- yeGFP (C terminal on insert)
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCustom Gene blocks through Twist Bioscience
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PAF24 was a gift from Ryan Peterson (Addgene plasmid # 223172 ; http://n2t.net/addgene:223172 ; RRID:Addgene_223172) -
For your References section:
Characterization of a High-Affinity Copper Transporter CTR1a in the White-Nose Syndrome Causing Fungal Pathogen Pseudogymnoascus destructans. Anne S, Friudenberg AD, Peterson RL. J Fungi (Basel). 2024 Oct 21;10(10):729. doi: 10.3390/jof10100729. 10.3390/jof10100729 PubMed 39452681