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pAAV-TNT4-Zfr-P2A-HA-luciferase
(Plasmid #223188)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223188 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    luciferase
  • Alt name
    fluc
  • Alt name
    Photinus pyralis luciferase
  • Alt name
    firefly luciferase
  • Species
    firefly
  • Entrez Gene
    LOC116160065 (a.k.a. PPYR_00001, LUC1, PpyLuc1, luc, luciferase)
  • Promoter TNNT2
  • Tags / Fusion Proteins
    • engineered P2A (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGCATTCCGTCAGATACACAAAGTgctactaacttcagcctActAaaAcaggctggagacgtTgaggagaaccctggacctTATCCATAcGACGTTCCAGATTACGCagaag
  • 3′ sequencing primer gcggaaagatgccgtgtaaGTACCTCTAGAGTCGACCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-TNT4-Zfr-P2A-HA-luciferase was a gift from Yuxuan Guo (Addgene plasmid # 223188 ; http://n2t.net/addgene:223188 ; RRID:Addgene_223188)

  • For your References section:

    The drug-elicitable alternative splicing module for tunable vector expression in the heart. Chen Z, Yang L, Zhang Y, Li J, Yang Y, Li Y, Fan L, Chen W, Miao L, Liu J, Chen G, Wang Z, Li Y, Gao F, Zhou J, Zheng L, Zhang Y, Zhao D, Pu WT, Yang K, Dong E, Guo Y. Nat Cardiovasc Res. 2025 Jun 13. doi: 10.1038/s44161-025-00665-7. 10.1038/s44161-025-00665-7 PubMed 40514436
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