pCXLE-hTBX3
(Plasmid
#223196)
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PurposeEpisomal plasmid expresses hTBX3
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223196 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepCXLE-gw
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Backbone manufacturerShinya Yamanaka
- Backbone size w/o insert (bp) 11898
- Total vector size (bp) 14130
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameT-Box Transcription Factor 3
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Alt nameTBX3
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SpeciesH. sapiens (human)
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Insert Size (bp)2232
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GenBank IDNM_005996.4 NM_005996.4
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Entrez GeneTBX3 (a.k.a. TBX3-ISO, UMS, XHL)
- Promoter CAG
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pCAG-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCXLE-hTBX3 was a gift from R. Michael Roberts (Addgene plasmid # 223196 ; http://n2t.net/addgene:223196 ; RRID:Addgene_223196)