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Addgene

CMV_Mex3a_mutRING_t2a_mCherry
(Plasmid #223245)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223245 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRE2
  • Total vector size (bp) 5777
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mex3a
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1560
  • Mutation
    4 point mutations in RING Domain: Cys 484 Ala; His 486 Ala; Cys 490 Ala; Cys 493 Ala
  • Entrez Gene
    Mex3a (a.k.a. 2700083E18Rik, Gm411, Rkhd4)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note, this mutantRING version of Mex3a preserves mus musculus amino acids for Mex3a (except the four amino acids of RING domain's cross brace structure), but changes the underlying DNA sequence to reduce CG content.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV_Mex3a_mutRING_t2a_mCherry was a gift from Stavros Lomvardas (Addgene plasmid # 223245 ; http://n2t.net/addgene:223245 ; RRID:Addgene_223245)
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