pBA1370_pTet-ECOR31_Hachiman
(Plasmid
#223364)
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PurposeExpression of ECOR31 Hachiman under the pTet promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 223364 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonep15a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameECOR31 Hachiman
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SpeciesE. coli ECOR31
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.02.29.582594 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBA1370_pTet-ECOR31_Hachiman was a gift from Jennifer Doudna (Addgene plasmid # 223364 ; http://n2t.net/addgene:223364 ; RRID:Addgene_223364) -
For your References section:
Genome integrity sensing by the broad-spectrum Hachiman antiphage defense complex. Tuck OT, Adler BA, Armbruster EG, Lahiri A, Hu JJ, Zhou J, Pogliano J, Doudna JA. Cell. 2024 Nov 27;187(24):6914-6928.e20. doi: 10.1016/j.cell.2024.09.020. Epub 2024 Oct 11. 10.1016/j.cell.2024.09.020 PubMed 39395413