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pBA1469_pTet-ECOR31Ham_hamA_E138A_K140A_Mut
(Plasmid #223368)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223368 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    p15a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ECOR31 Hachiman mutant (HamA E138A, K140A)
  • Species
    E. coli ECOR31
  • Mutation
    HamA E138A, K140A

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.02.29.582594 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBA1469_pTet-ECOR31Ham_hamA_E138A_K140A_Mut was a gift from Jennifer Doudna (Addgene plasmid # 223368 ; http://n2t.net/addgene:223368 ; RRID:Addgene_223368)

  • For your References section:

    Genome integrity sensing by the broad-spectrum Hachiman antiphage defense complex. Tuck OT, Adler BA, Armbruster EG, Lahiri A, Hu JJ, Zhou J, Pogliano J, Doudna JA. Cell. 2024 Nov 27;187(24):6914-6928.e20. doi: 10.1016/j.cell.2024.09.020. Epub 2024 Oct 11. 10.1016/j.cell.2024.09.020 PubMed 39395413
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