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pBA1583_ECOR31HamA(E138AK140A)B_CoPurification
(Plasmid #223372)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223372 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMBP
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MBP-His-TEV-ECOR31 Hachiman mutant (HamA E138A, K140A)
  • Species
    E. coli ECOR31
  • Mutation
    HamA E138A, K140A
  • Tag / Fusion Protein
    • MBP-His-TEV (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Only HamA is tagged. HamB is pulled down during HamA purification.

Please visit https://doi.org/10.1101/2024.02.29.582594 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBA1583_ECOR31HamA(E138AK140A)B_CoPurification was a gift from Jennifer Doudna (Addgene plasmid # 223372 ; http://n2t.net/addgene:223372 ; RRID:Addgene_223372)

  • For your References section:

    Genome integrity sensing by the broad-spectrum Hachiman antiphage defense complex. Tuck OT, Adler BA, Armbruster EG, Lahiri A, Hu JJ, Zhou J, Pogliano J, Doudna JA. Cell. 2024 Nov 27;187(24):6914-6928.e20. doi: 10.1016/j.cell.2024.09.020. Epub 2024 Oct 11. 10.1016/j.cell.2024.09.020 PubMed 39395413
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