CMV-SNAP-hCB2_IRES_EGFP
(Plasmid
#223506)
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PurposeExpression of CB2 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker
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Depositing Lab
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Depositing OrganizationOregon Health & Science University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 223506 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 223506-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 223506-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepIRES2
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Backbone manufacturerClontech (TaKaRa)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSNAP-tag-CNR2 and EGFP
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SpeciesH. sapiens (human), Synthetic
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Entrez GeneCNR2 (a.k.a. CB-2, CB2, CX5)
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Tag
/ Fusion Protein
- SNAP-tag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 223506-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 223506-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-SNAP-hCB2_IRES_EGFP was a gift from James Frank (Addgene plasmid # 223506 ; http://n2t.net/addgene:223506 ; RRID:Addgene_223506) -
For your References section:
Platform Reagents Enable Synthesis of Ligand-Directed Covalent Probes: Study of Cannabinoid Receptor 2 in Live Cells. Kosar M, Sykes DA, Viray AEG, Vitale RM, Sarott RC, Ganzoni RL, Onion D, Tobias JM, Leippe P, Ullmer C, Zirwes EA, Guba W, Grether U, Frank JA, Veprintsev DB, Carreira EM. J Am Chem Soc. 2023 Jul 19;145(28):15094-15108. doi: 10.1021/jacs.2c13629. Epub 2023 Jul 4. 10.1021/jacs.2c13629 PubMed 37401816
