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pHR TRE_SEAP_IRES_mCherry-PGK_BFP-hf
(Plasmid #223535)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223535 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR
  • Total vector size (bp) 12961
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Secreted alkaline phosphatase
  • Alt name
    SEAP
  • Insert Size (bp)
    1563

Gene/Insert 2

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    708

Gene/Insert 3

  • Gene/Insert name
    TagBFP
  • Alt name
    Blue fluorescent protein
  • Insert Size (bp)
    702

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.01.31.578281 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR TRE_SEAP_IRES_mCherry-PGK_BFP-hf was a gift from Jonathan Brunger (Addgene plasmid # 223535 ; http://n2t.net/addgene:223535 ; RRID:Addgene_223535)

  • For your References section:

    A programmable arthritis-specific receptor for guided articular cartilage regenerative medicine. Walton BL, Shattuck-Brandt R, Hamann CA, Tung VW, Colazo JM, Brand DD, Hasty KA, Duvall CL, Brunger JM. Osteoarthritis Cartilage. 2025 Feb;33(2):231-240. doi: 10.1016/j.joca.2024.12.002. Epub 2024 Dec 18. 10.1016/j.joca.2024.12.002 PubMed 39706287
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