pHR Ef1a_LaG16-synNotch_tTA-PGK_Puro
(Plasmid
#223536)
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PurposeAnti-GFP LaG16 nanobody-based synNotch; puro-selectable
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223536 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
- Total vector size (bp) 13029
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLaG16_synNotch_tTA
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SpeciesSynthetic
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Insert Size (bp)2223
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Tag
/ Fusion Protein
- c-myc tag
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.01.31.578281 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR Ef1a_LaG16-synNotch_tTA-PGK_Puro was a gift from Jonathan Brunger (Addgene plasmid # 223536 ; http://n2t.net/addgene:223536 ; RRID:Addgene_223536) -
For your References section:
A programmable arthritis-specific receptor for guided articular cartilage regenerative medicine. Walton BL, Shattuck-Brandt R, Hamann CA, Tung VW, Colazo JM, Brand DD, Hasty KA, Duvall CL, Brunger JM. Osteoarthritis Cartilage. 2025 Feb;33(2):231-240. doi: 10.1016/j.joca.2024.12.002. Epub 2024 Dec 18. 10.1016/j.joca.2024.12.002 PubMed 39706287