hSyn1-mCherry-CAAX
(Plasmid
#223674)
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PurposeAAV expression of mCherry from hSyn1 promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223674 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepsynapsin
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry-CAAX
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SpeciesSynthetic
- Promoter hSyn1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site EcoRV (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hSyn1-mCherry-CAAX was a gift from Jun Nagai (Addgene plasmid # 223674 ; http://n2t.net/addgene:223674 ; RRID:Addgene_223674) -
For your References section:
The astrocytic ensemble acts as a multiday trace to stabilize memory. Dewa K, Kaseda K, Kuwahara A, Kubotera H, Yamasaki A, Awata N, Komori A, Holtz MA, Kasai A, Skibbe H, Takata N, Yokoyama T, Tsuda M, Numata G, Nakamura S, Takimoto E, Sakamoto M, Ito M, Masuda T, Nagai J. Nature (2025) 10.1038/s41586-025-09619-2