pHBlaM_GFP
(Plasmid
#223809)
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Purposeinducible expression of high-copy BlaM (cytoplasmic variant) and constitutive expression of GFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223809 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepHSG298
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Backbone manufacturerTakara Bio
- Backbone size w/o insert (bp) 2675
- Total vector size (bp) 4857
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namesuperfolder GFP
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Alt namesfGFP
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Insert Size (bp)714
Gene/Insert 2
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Gene/Insert nameBlaM
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Insert Size (bp)795
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHBlaM_GFP was a gift from Lingchong You (Addgene plasmid # 223809 ; http://n2t.net/addgene:223809 ; RRID:Addgene_223809) -
For your References section:
Private benefit of beta-lactamase dictates selection dynamics of combination antibiotic treatment. Ma HR, Xu HZ, Kim K, Anderson DJ, You L. Nat Commun. 2024 Sep 27;15(1):8337. doi: 10.1038/s41467-024-52711-w. 10.1038/s41467-024-52711-w PubMed 39333122