pET50del_SynPspA
(Plasmid
#223818)
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PurposeBacterial expression of SynPspA with N-terminal 6xHis-Tag and 3C cleavage site
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223818 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepET50
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSynPspA
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Alt namePspA
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SpeciesSynechocystis sp. PCC6803
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET50del_SynPspA was a gift from Carsten Sachse (Addgene plasmid # 223818 ; http://n2t.net/addgene:223818 ; RRID:Addgene_223818) -
For your References section:
Structural plasticity of bacterial ESCRT-III protein PspA in higher-order assemblies. Junglas B, Hudina E, Schonnenbeck P, Ritter I, Heddier A, Santiago-Schubel B, Huesgen PF, Schneider D, Sachse C. Nat Struct Mol Biol. 2025 Jan;32(1):23-34. doi: 10.1038/s41594-024-01359-7. Epub 2024 Aug 16. 10.1038/s41594-024-01359-7 PubMed 39152237