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Addgene

pET50del_SynPspA_R44K/E126Q/E179Q
(Plasmid #223819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223819 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET50
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SynPspA
  • Alt name
    PspA
  • Species
    Synechocystis sp. PCC6803
  • Mutation
    R44K, E126Q, E179Q
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET50del_SynPspA_R44K/E126Q/E179Q was a gift from Carsten Sachse (Addgene plasmid # 223819 ; http://n2t.net/addgene:223819 ; RRID:Addgene_223819)

  • For your References section:

    Structural plasticity of bacterial ESCRT-III protein PspA in higher-order assemblies. Junglas B, Hudina E, Schonnenbeck P, Ritter I, Heddier A, Santiago-Schubel B, Huesgen PF, Schneider D, Sachse C. Nat Struct Mol Biol. 2025 Jan;32(1):23-34. doi: 10.1038/s41594-024-01359-7. Epub 2024 Aug 16. 10.1038/s41594-024-01359-7 PubMed 39152237
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