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Addgene

pMSP1D1-Strep-II
(Plasmid #224209)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224209 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET 28a
  • Total vector size (bp) 5883
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MSP1D1-Strep-II
  • Alt name
    Membrane scaffold protein
  • Species
    Synthetic
  • Mutation
    Substitution of 7xHis tag found in pMSP1D1 for a Strep-II tag
  • Promoter T7
  • Tag / Fusion Protein
    • Strep-II Tag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pMSP1D1(#20061) was obtained from Addgene (Stephen Sligar). The N-term 7xHis tag was replaced with a Strep-II tag.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSP1D1-Strep-II was a gift from Anne Robinson (Addgene plasmid # 224209 ; http://n2t.net/addgene:224209 ; RRID:Addgene_224209)

  • For your References section:

    Ligand binding kinetics to evaluate the function and stability of A(2A)R in nanodiscs. Pettersen JM, McCracken O, Robinson AS. Biophys J. 2025 Jan 21;124(2):440-457. doi: 10.1016/j.bpj.2024.12.018. Epub 2024 Dec 17. 10.1016/j.bpj.2024.12.018 PubMed 39690743
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