GfaABC1D-mGrm3-WPRE
(Plasmid
#224236)
-
PurposeAAV expression of mGrm3 from GfaABC1D promoter
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 224236 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepAAV
-
Vector typeMammalian Expression, AAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemGrm3
-
SpeciesM. musculus (mouse)
-
Entrez GeneGrm3 (a.k.a. 0710001G23Rik, Gprc1c, mGlu3, mGluR3)
- Promoter GfaABC1D
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoNI (unknown if destroyed)
- 3′ cloning site AccI (unknown if destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
GfaABC1D-mGrm3-WPRE was a gift from Jun Nagai (Addgene plasmid # 224236 ; http://n2t.net/addgene:224236 ; RRID:Addgene_224236) -
For your References section:
The astrocytic ensemble acts as a multiday trace to stabilize memory. Dewa K, Kaseda K, Kuwahara A, Kubotera H, Yamasaki A, Awata N, Komori A, Holtz MA, Kasai A, Skibbe H, Takata N, Yokoyama T, Tsuda M, Numata G, Nakamura S, Takimoto E, Sakamoto M, Ito M, Masuda T, Nagai J. Nature (2025) 10.1038/s41586-025-09619-2