pAM75
(Plasmid
#224249)
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PurposepET28a MWG-ubiquitin-G77-C78
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 224249 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMWG-ubiquitin-G77-C78
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SpeciesS. cerevisiae (budding yeast)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAM75 was a gift from Andreas Martin (Addgene plasmid # 224249 ; http://n2t.net/addgene:224249 ; RRID:Addgene_224249) -
For your References section:
High-Throughput Assay for Characterizing Rpn11 Deubiquitinase Activity. Xie G, Dong KC, Worden EJ, Martin A. Methods Mol Biol. 2023;2591:79-100. doi: 10.1007/978-1-0716-2803-4_6. 10.1007/978-1-0716-2803-4_6 PubMed 36350544