p3E-IRES-(n2)StayGold(c4)-pA (JDW 1364)
              
              
                (Plasmid
                
                #224537)
              
            
            
            
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            PurposeA Gateway compatible 3' entry clone containing IRES-(n2)StayGold(c4)-pA
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              Depositing Lab
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          Sequence Information
 
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 224537 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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            Vector backbonepDONR P2R P3
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              Vector typeGateway cloning
 
Growth in Bacteria
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            Bacterial Resistance(s)Kanamycin, 50 μg/mL
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            Growth Temperature37°C
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            Growth Strain(s)DH5alpha
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            Copy numberHigh Copy
 
Gene/Insert
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                Gene/Insert nameIRES-(n2)StayGold(c4)-pA
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                    SpeciesSynthetic
 
Cloning Information
- Cloning method Gateway Cloning
 
Resource Information
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            Supplemental Documents
 
Terms and Licenses
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        Academic/Nonprofit Terms
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      Industry Terms
- Not Available to Industry
 
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
 
Depositor Comments
Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3E-IRES-(n2)StayGold(c4)-pA (JDW 1364) was a gift from Joshua Wythe (Addgene plasmid # 224537 ; http://n2t.net/addgene:224537 ; RRID:Addgene_224537) - 
                
For your References section:
Multisite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox with diverse applications in vertebrate model systems. Gillespie W, Zhang Y, Ruiz OE, Cerda J, Ortiz-Guzman J, Turner WD, Largoza G, Sherman M, Mosser LE, Fujimoto E, Chien CB, Kwan KM, Arenkiel BR, Devine WP, Wythe JD. bioRxiv [Preprint]. 2024 Jul 13:2024.07.13.603267. doi: 10.1101/2024.07.13.603267. 10.1101/2024.07.13.603267 PubMed 39026881