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pcDNA3.1-Pur-BFP-P2A-otMx1
(Plasmid #224553)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224553 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1-Pur-(-)
  • Backbone size w/o insert (bp) 5528
  • Total vector size (bp) 8258
  • Modifications to backbone
    Backbone = Addgene #200458
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BFP-P2A-otMx1
  • Species
    Oncorhynchus tshawytscha
  • Insert Size (bp)
    2730
  • Entrez Gene
    LOC112247236
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GGCTAACTAGAGAACCCACTG
  • 3′ sequencing primer GGCAACTAGAAGGCACAGTC
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-Pur-BFP-P2A-otMx1 was a gift from Bertrand Collet (Addgene plasmid # 224553 ; http://n2t.net/addgene:224553 ; RRID:Addgene_224553)

  • For your References section:

    Knockout of the antiviral genes mx1 or mx3 modulates the expression of paralogous genes in a salmonid cell line. Chaumont L, Peruzzi M, Huetz F, Raffy C, Le Hir J, Leong JC, Boudinot P, Collet B. Fish & Shellfish Immunology, 09 Feb 2026; doi: 10.1016/j.fsi.2026.111204 10.1016/j.fsi.2026.111204
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