pUBC(h)-DEST-mKOk
(Plasmid
#224830)
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Purpose(Empty Backbone) Empty Gateway DEST vector for expressing C-terminal tagged proteins with mKusabiraOrangekand hygromycin selection in plants
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224830 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepUBC(h)-DEST-mKOk
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Vector typePlant Expression
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Tag
/ Fusion Protein
- mKusabiraOrangek (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- mKusabiraOrangek (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.06.20.660776 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUBC(h)-DEST-mKOk was a gift from Rebecca Roston (Addgene plasmid # 224830 ; http://n2t.net/addgene:224830 ; RRID:Addgene_224830) -
For your References section:
Lipid Metabolism and Contact Site Homologs Are Enriched at the Chloroplast Envelope-Thylakoid Interface. LaBrant EW, Smith CN, Torres-Gerena AD, Ishimwe J, Huang F, Tullis A, Litterer L, Modi B, Naldrett MJ, Altartouri B, Roston RL. bioRxiv 2025.06.20.660776; doi: https://doi.org/10.1101/2025.06.20.660776 10.1101/2025.06.20.660776