Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22502||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 10000
Vector typeMammalian Expression
Growth in Bacteria
Growth instructionsTransform into DH5alpha and grow in Luria Broth containing 100µg/ml ampicillin.
Copy numberHigh Copy
Gene/Insert nameTransactivator of Transcription
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byOriginal plasmid sent by Jason Coleman and Matthew Huentelman.
Terms and Licenses
This plasmid forms part of a plasmid set for lentivirus generation in the Kasparov lab, University of Bristol, UK.
Diagnostic digest: Digest with HindIII, expect 0.25 and 10 kb fragments.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCEP4-tat was a gift from Sergey Kasparov (Addgene plasmid # 22502 ; http://n2t.net/addgene:22502 ; RRID:Addgene_22502)
For your References section:Efficient large-scale production and concentration of HIV-1-based lentiviral vectors for use in vivo. Coleman JE, Huentelman MJ, Kasparov S, Metcalfe BL, Paton JF, Katovich MJ, Semple-Rowland SL, Raizada MK. Physiol Genomics. 2003 Feb 6. 12(3):221-8. 10.1152/physiolgenomics.00135.2002 PubMed 12488511