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Addgene

PB-TRE-ETV2
(Plasmid #225051)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225051 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB-TRE3G
  • Backbone size w/o insert (bp) 8075
  • Total vector size (bp) 9104
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ETS Variant Transcription Factor 2
  • Alt name
    ETV2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1029
  • Mutation
    Codon Optimized
  • GenBank ID
    NP_001287903.1
  • Entrez Gene
    ETV2 (a.k.a. ER71, ETSRP71)
  • Promoter TRE3G

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer GTACGGTGGGCGCCTATAAA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-TRE-ETV2 was a gift from Sean Palecek & Eric Shusta (Addgene plasmid # 225051 ; http://n2t.net/addgene:225051 ; RRID:Addgene_225051)

  • For your References section:

    ETV2 Overexpression Promotes Efficient Differentiation of Pluripotent Stem Cells to Endothelial Cells. Ding Y, Tamhankar S, Du F, Christopherson T, Schlueter N, Cohen JR, Shusta EV, Palecek SP. Biotechnol Bioeng. 2025 Jul;122(7):1914-1928. doi: 10.1002/bit.28979. Epub 2025 Mar 25. 10.1002/bit.28979 PubMed 40134129
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